Regulation on function and maturation of plasmacytoid dendritic cells by triptolide
10.3760/cma.j.issn.0254-5101.2011.01.002
- VernacularTitle:雷公藤内酯醇对浆细胞样树突状细胞功能及成熟的影响
- Author:
Limin LIU
;
Wenjing JIAO
;
Xingxia ZHANG
;
Yanming ZHANG
;
Guangsheng ZHAO
;
Hongxia CUI
;
Yumei SUN
;
Shuhua LU
- Publication Type:Journal Article
- Keywords:
Triptolide;
Plasmacytoid dendritic cells;
Maturation
- From:
Chinese Journal of Microbiology and Immunology
2011;31(1):6-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of immunomodulatory activity of triptolide on healthy volunteers peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDCs). Methods Healthy volunteers-derived pDCs were sorted by flow cytometry, then incubated with triptolide (0, 5, 10, 30 μg/L). After 24 hours, we detected the concentration of IFN-α, IL-6, TNF-α using ELISA. After 5 days, the cultrural cells were collected and analyzed by flow cytometry, light microscope and electron microscope scanning. Results Triptolide-treated pDCs secreted lower level of IFN-α,IL-6 ,TNF-α, triptolide could inhibit pDCs differentiation to DCs which displayed more immature morphology and immunophenotypes than untreated-pDCs. Conclusion Triptolide could decrease the immune function of pDCs, inhibit differentiation and maturation of pDCs.
