The expression of miRNAs in endoscopic ultrasound-guided fine needle pancreatic aspirates and its clinical significance
10.3760/cma.j.issn.0254-1432.2010.12.004
- VernacularTitle:超声内镜细针穿刺研究胰腺组织中微小核糖核酸的表达及临床意义
- Author:
Xiaowei WANG
;
Jun GAO
;
Jianqiang LIU
;
Yan REN
;
Zhendong JIN
;
Yiqi DU
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
MicroRNAs;
Endosonography
- From:
Chinese Journal of Digestion
2010;30(12):877-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miRNAs in endoscopic ultrasound (EUS)-guided fine needle pancreatic aspirates (FNA) and its clinical significance. Methods The expression of miRNA-210, miRNA-21, miRNA-196a, miRNA-181a, miRNA-181b, miRNA-155 and miRNA-16 in EUS-FNA specimen of 23 pancreatic cancer and 13 benign pancreatic masses was detected with quantitative real-time polymerase chain reaction (qRT-PCR) assays and its clinical significance was analyzed. Results The relatively expression quantity of miRNA-210, miRNA-21,miRNA-196a, miRNA-181a and miRNA-181b (0.86±1.10, 0.69±0.64, 0.32±2.50, 0.16±0.83and 0.56 ±0.88, respectively) was significant higher in pancreatic cancer than those in the benign pancreatic masses (-0.11±0.98,-0.03±0.97,-1.50±1.40,-0.53±1.10 and -0.28±1.10,respectively) (P value was 0. 012, 0. 011, 0. 024, 0. 036 and 0.015, respectively). The relatively expression quantity of miRNA-16 and miRNA-155 in pancreatic cancer and benign pancreatic masses was - 0.11 ± 0.69, 0.08 ± 1.04, - 0.73 ± 1.26 and - 0.19 ± 1.19 respectively, no statistic difference (P value was 0. 067 and 0. 467). The high expression of miRNA196a was positively correlated with pancreatic cancer lymphatic metastasis and clinical TNM staging. Conclusion There were multi miRNAs with abnormal high expression in the pancreatic cancer. Of those, miRNA-196a was associated with the clinical malignant features of pancreatic cancer.
