Quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa induces the apoptosis of Caco-2 cells
10.3760/cma.j.issn.0254-5101.2010.06.003
- VernacularTitle:铜绿假单胞菌密度感应系统自体诱导因子3OC12-HSL对人肠道上皮细胞Caco-2凋亡的影响及其机制
- Author:
Lu YE
;
Hongtao LI
;
Lili WANG
;
Mingyou XING
;
Jianxin SONG
- Publication Type:Journal Article
- Keywords:
Quorum sensing system;
3OC12-HSL;
Apoptosis p38/MAPK;
Caco-2 cells
- From:
Chinese Journal of Microbiology and Immunology
2010;30(6):506-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa on the apoptosis of Caco-2 cells and its mechanism. Methods Caco-2 cells were incubated with 3OC12-HSL for 4 h and then examined by MTT method for cytoactivities. Flow cytometry was used to analyze apoptosis rate of Caco-2 cells. Expression of apoptosis associated proteins p-p38/MAPK and NF-κB were detected by Western blot. Results After exposure to 3OC12-HSL for 4 h, cytoactivities of Caco-2 cells was reduced(P<0.05) with dose-dependent pattern, and higher dose of 3OC12-HSL leaded to increasing apoptosis rate of Caco-2 cells(P<0.05). 3OC12-HSL raised expression of apoptosis associated proteins p-p38/MAPK and NF-κB detected by Western blot. Conclusion Quorum sensing autoinducer 3OC12-HSL can effect cytoactivities of Caco-2 cells and may induce its apoptosis by enhancing the expression of p-p38/MAPK and NF-κB protein.
