Characterization of immune response in mice induced by recombinant vaccinia virus(Tiantan) based multivalent H5N1 avian influenza vaccines
10.3760/cma.j.issn.0254-5101.2010.06.014
- VernacularTitle:人禽流感病毒H5N1多价重组痘苗病毒(天坛株)疫苗在小鼠中的免疫原性研究
- Author:
Wen WANG
;
Yao DENG
;
Wenjie TAN
;
Hong CHEN
;
Min WANG
;
Xiao YIN
;
Jie GUAN
;
Jianfang ZHOU
;
Yuelong SHU
;
Li RUAN
- Publication Type:Journal Article
- Keywords:
Highly pathogenic avian influenza (HPAI) virus;
H5N1;
Recombinant vaccinia (Tiantan) virus;
Live viral vector-based vaccine
- From:
Chinese Journal of Microbiology and Immunology
2010;30(6):546-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an effective and broad immune protective H5N1 vaccine.Methods We first developed two recombinant vaccinia ( Tiantan strain) virus ( rTTV ) based H5N1 vaccines, which consisted of bicistron expressing the hemagglutinin(HA) and matrix protein 2(M2), or bicistron expressing the neuraminidase(NA) and matrix protein 1 (M1). The expression of H5N1 protein in rTTVs was confirmed. We immunized the BALB/c mice twice with two kind of dose ( 104 PFU, 107 PFU)using different combination. Subsequently, we assessed the humoral and cellular immune response in vaccinated mice. Results Our data showed that rTTV-based H5N1 vaccine induced rapidly robust HA- and NAspecific antibody level and IFN-γ secreting form cell(SFC) with either single dose of 107 PFU or twice dose of 104 PFU or 107 PFU. We also detected significant neutralizing antibody and matrix-specific immune response. In addition, we found that immunization with two kind of rTTV-based H5N1 vaccines induced much high level of M2-specific antibody than that with single of rTTV-based H5N1 vaccine. Conclusion rTTVbased H5N1 vaccines in this study elicited board array of immunity and our study offers a promising alternative H5N1 vaccine candidates with favorable potential to prevent various H5N1 pandemic.
