Detection of mdr-1, P-gp and DNA ploidy in neo-adjuvant chemotherapy of invasive bladder carcinoma

VernacularTitle:多药耐药基因、P-糖蛋白及DNA倍体检测在浸润性膀胱癌新辅助化疗中的意义
Author: Jin WANG ; Haichao LI ; Rongjun LI
Publication Type:Journal Article
Keywords: Urinary bladder neoplasms; Multidrug resistance-associated proteins; P-glycoprotein; DNA heteroploidy
From: Cancer Research and Clinic 2010;22(9):619-621
CountryChina
Language:Chinese
Abstract: Objective To explore the significance on expression of mdr-1, P-gp and DNA heteroploidy in invasive bladder carcinoma. Methods The expressions of mdr-1 and P-gp were detected by RT-PCR and immunohistochemistry(SP) method, and DNA ploidy by flow cytometry method in 26 cases of invasive bladder carcinoma tissues, which were compared with those in 10 normal bladder tissues. At the same time, the flow cytometry was used to determine DNA heteroploidy. Results A of mdr-1 in the neoadjuvant chemotherapy CR, PR, non-respond and control group were (0.61±0.75), (0.71±0.11), (1.68±0.28) and (0.745±0.13),respectively (P＜0.05). Positive rates of P-gp had significant differences between CR, PR and non-respondgroup and control group (P ＜0.05). Positive rates of DNA heteroploid in the above groups were 50 %, 45 %,69 % and 10 %, respectively (P ＜0.05). Conclusion The detections of mdr-1, P-gp and of DNA heteroploid contribute to evaluate neo-adjuvant chemotherapy effects of invasive bladder carcinoma.