Identification and priliminary application of monoclonal antibodies against inrisive Aspergillus
10.3760/cma.j.issn.1009-9158.2010.09.017
- VernacularTitle:侵袭性曲霉感染特异性抗体的筛选及其初步临床应用研究
- Author:
Lingxiao JIANG
;
Yanfang WANG
;
Wei HAO
;
Liwen QIU
;
Jianpiao CAI
;
Yuxian PAN
;
Wenxia CHEN
;
Changhong JIANG
;
Lijuan LIN
;
Xiaoyan CHE
- Publication Type:Journal Article
- Keywords:
Aspergillus;
Iinfection;
Diagnosis;
Biomarker
- From:
Chinese Journal of Laboratory Medicine
2010;33(9):884-890
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen monoclonal antibodies (mAbs) for early diagnosis of invisive Aspergillus. Methods Monoclonal antibodies against different antigens of Aspergillus fumigatus were produced. The two pairs of combinations of monoclonal antibodies were selected accoring the distinct epitopes and double-antibody sandwich ELISA based on mAbs above were established. The sensitivity and specificity of the methods were analyzed by detecting culture supernatants of clinical isolates and environmental isolatesof Aspergillus. spp, Penicillium Marneffei, Candidas, and serum from animal models and patients. The epitopes recognized by mAbs were identified by immunobotting. Results A total of 32 hybridoma cell lines that stably produced MAbs were obtained. Two double- antibody sandwich ELISAs were established. One method was specific for 19 clinical isolates and environmental isolates of Aspergillus. spp, whereas the other one was specific for the clinical and environmental isolates of Aspergillus fumigatus without cross-reation with other Aspergillus. spp. For the same kind of medium of Aspergillus fumigatus, the sensitivity of the first method was 10 fold higher than the second method. Conclusions The specific mAbs for early diagnosis of invisive Aspergillus were obtained. Antigen recognized by the specific mAbs was mannoprotein with molecular weights of approximately 25 000-75 000. This antigen was potential early diagnostic marker for invasive Aspergillus.
