Modulation of macrophage function stimulated with oxidized low density lipoprotein by CD4+CD25+regulatory T cells

VernacularTitle:CD4+CD25+调节性T细胞对氧化型低密度脂蛋白活化的巨噬细胞功能的影响
Author: Ming LI ; Dazhu LI ; Jing LIN ; Shaolin HE ; Xuming MA
Publication Type:Journal Article
Keywords: CD4 + CD25 + Tr; Macrophage heterogeneity; Atherosclerosis
From: Chinese Journal of Microbiology and Immunology 2010;30(12):1124-1127
CountryChina
Language:Chinese
Abstract: Objective To investigate whether and how CD4 + CD25 + regulatory T cells(Tr) affect oxidized low density lipoprotein(oxLDL) induced proinflammatory response in macrophages.Methods Tr were isolated from lymphocyte suspensions by magnetic cell sorting-column and analyzed by flow cytometry.Macrophages were cultured alone,with CD4 + CD25 + Tr or CD4 + CD25 - Tr in the presence of oxLDL for 48 h.The phenotype of macrophages was determined by flow cytometry.NO production was assessed by Griess reaction an iNOS mRNA was isolated by RT-PCR.ELISA were used to measure the production of cytokine/chemokine like MCP-1,MMP-9,TNF-ct,TGF-β and IL-10 in macrophages response to oxLDL.Results Our data showed that with oxLDL challenge,the Tr modulated macrophages have decreased NO production and iNOS expression,decreased HLA-DR and CD86 expression,and down-regulated proinflammatory cytokine/chemokine production.Conclusion Tr can inhibit the proinflammatory properties of macrophages and steer macrophage differentiation toward an anti-inflammatory cytokine producing phenotype.