Regulatory effect of LPS on interleukin 27 and its relationship between COX-2 and PGE2

VernacularTitle:脂多糖对白细胞介素27的调节作用及其与环氧化酶2及前列腺素E2之间的相关性研究
Author: Chengliang ZHU ; Yan LI ; Rui ZHANG ; Shiyong HAO ; Pingan ZHANG ; Fang LIU
Publication Type:Journal Article
Keywords: Interleukin 27; COX-2; PGE2
From: Chinese Journal of Microbiology and Immunology 2010;30(12):1133-1136
CountryChina
Language:Chinese
Abstract: Objective To explore the regulatory effect of LPS on the expression of interleukin 27 (IL-27),and uncover the relationships among cyclooxyenase-2(COX-2),prostaglandin E2(PGE2) and IL-27.Methods THP-1 cells were stimulated with different doses of LPS,IL-27 and PGE2 levels in the supernatants were measured by enzyme-linked immunosorbent assay(ELISA) at different time,expression of COX-2 was measured by RT-PCR and Western blot.Results IL-27 can be induced by LPS in THP-1 cells in a time and dose dependent fashion,and IL-27 induces COX-2 mRNA expression,COX-2 protein production,and PGE2 release in a time and dose fashion.Conclusion LPS can stimulate the relase of IL-27 at cell level,and IL-27 can induce the expression of COX-2 and production of PGE2.