Sequence analysis and genotyping of genital Chlamydia trachomatis among patients with suspected-Neisseria gonorrhoeae
10.3760/cma.j.issn.0254-5101.2010.12.022
- VernacularTitle:拟诊为淋病患者中泌尿生殖道沙眼衣原体基因分型及序列分析
- Author:
Juanjuan ZHANG
;
Guanglu ZHAO
;
Lijun ZHANG
;
Feng WANG
;
Fuchang HONG
;
Lina LAN
;
Xiaobing WU
;
Xiaohua TAO
;
Chunlai ZHANG
;
Ciyong LU
;
Tiejian FENG
- Publication Type:Journal Article
- Keywords:
Gonorrhea;
Chlamydia trachomatis;
Nested-PCR;
Genotyping;
Sequencing analysis
- From:
Chinese Journal of Microbiology and Immunology
2010;30(12):1159-1163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the prevalence rate of genital Chlamydia trachomatis among a population with suspected-Neisseria gonorrhoeae infection,the distribution of Chlamydia trachomatis genotypes,assess changes in omp1 sequences among patients with Neisseria gonorrhoeae and Chlamydia trachomatis coinfections.Methods Four hundred and one swabs were collected.Chlamydia trachomatis and Neisseria gonorrhoeae were detected by Roche Amplicor System.DNA were extracted from those samples and were amplified by nested PCR.PCR products were sequencing and analyzed by software Mega4.0.Results The prevalence of genital Chlamydia trachomatis infection,Neisseria gonorrhoeae infection and coinfection with genital gonorrhoea and genital chlamydia were 82.3%,24.2% and 21.7% each.Eight genotypes were identified in 73 sequences,including E(27.4%),G/Ga(23.3%),D/Da(16.4%),F(13.7%),J (11.0%),H(5.5%),B and K(each 1.4%).Sequencing analysis showed that 3 cases(4.1%) had missense mutation,including genotype D/Da,E,G/Ga.Genotypes F,H,J and K were more variable,however,most of them were silent mutation.Conclusion The prevalence rate of genital Chlamydia trachomatis among a population with Neisseria gonorrhoeae infection was high.The most common genotypes were genotype E,G/Ga,D/Da and F; Sequencing analysis has provided a tool for the molecular epidemiology of genital Chlamydia trachomatis infections.
