Effect of siRNAs on HSV-1 Plaque Formation and Relative Expression Levels of RR mRNA
10.1007/s12250-011-3162-9
- Author:
Zhe REN
;
Shen LI
;
Qiaoli WANG
;
Yangfei XIANG
;
Yunxia CUI
;
Yifei WANG
;
Renbin QI
;
Daxiang LU
;
Shumin ZHANG
;
Peizhuo ZHANG
- Publication Type:Journal Article
- Keywords:
Small interfering RNA(siRNA);
Herpes simplex virus type Ⅰ(HSV-1);
Ribonucleotide reductase(RR);
Gene
- From:
Virologica Sinica
2011;26(1):40-46
- CountryChina
- Language:Chinese
-
Abstract:
RNA interference(RNAi)is a process by which introduced small interfering RNA(siRNA)can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1(HSV-1)RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
