Progenitor cells in mammalian maxillofacial and mandibular processes Source and differentiation phenotype
10.3969/j.issn.1673-8225.2010.50.044
- VernacularTitle:哺乳动物颌突前体细胞的来源及其分化表型
- Author:
Zeyuan ZHOU
;
Yan JIN
;
Shuxiang YU
;
Junnan SHI
;
Jianping ZHANG
;
Xiaoyan DUAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(50):9493-9496
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: It remains unclear whether ectomesenchymal cells also derived from neural crest stem cell in mammals.OBJECTIVE: To understand the specific markers and differentiation directions of maxillofacial and mandibular processes progenitor cells,and to explore the source and differentiation phenotype of ectomesenchymal stem cells.METHODS: The expression and changes of expression profiles of rat ectomesenchymal cells at E9.5,E10.5,E11.5,and E12.5days were observed by immunohistochemistry and flow cytometry.RESULTS AND CONCLUSION: The progenitors expressed multi-lineage markers,including neural system and several rnesenchymal tissue types,importantly the facts that molecule profiles were changed with time prolonged,suggesting these progenitors were in active differentiating stage,so they were stem like cells or contain stem like cells.Moreover,small populations(2%-3%)of CD57 and P75 phenotypes were detected by flow cytornetry,suggesting that ectomesenchymal stem cells were derived from neural crest,which maintained a quantitative stabilization though it is gradually differentiate after localization.
