The immunological effect of group A and C meningococcal conjugate vaccine with double protein carriers

VernacularTitle:双蛋白载体A、C群脑膜炎球菌多糖结合物的免疫效果观察
Author: Qiming LI ; Zhijing MA ; Jing ZHANG ; Yuqin JIN ; Shi CHEN ; Xuefeng ZHANG ; Meiying LIU
Publication Type:Journal Article
Keywords: Recombinant Escherichia coli heat-labile enterotoxin B subunit (rLTB); Polysaccharide conjugate vaccine; Group A and C meningococcal polysaccharides
From: Chinese Journal of Microbiology and Immunology 2011;31(2):146-149
CountryChina
Language:Chinese
Abstract: Objective To evaluate the immunogenicity of group A and C meningococcal polysaccharides conjugates using different proteins as carriers. Methods Heat-labile enterotoxin B subunit (LTB)pentamer form was expressed in E. coli. The target protein was identified and purified by cation-exchange chromatography. Then biological activity of rLTB was tested using GM1-ELISA. GCMP was conjugated to rLTB with the chemical method (ADH). Furthermore, the mice were immunized with GAMp-TT/GCMP-TT conjugates and GAMP-TT/GCMP-rLTB conjugates via peritoneal. Finally the anti-polysaccharide antibody was detected. Results The GAMP-TT/GCMP-rLTB conjugate elicits remarkably higher serum antibodies in mice than GAMP-TT/GCMP-TT conjugate. Conclusion These results indicated that polysaccharide conjugates using different proteins as carriers were superior to those using only one protein as carrier.