Comparision and application of detective methods for Her-2 gene in invasive breast carcinoma tissue
10.3760/cma.j.issn.1006-9801.2011.02.007
- VernacularTitle:乳腺浸润性导管癌组织表皮生长因子受体基因检测方法的比较及应用
- Author:
Dongmei ZHOU
;
Gang CHEN
;
Xiongwei ZHENG
;
Tianrong ZHENG
- Publication Type:Journal Article
- Keywords:
Carcinoma,ductal,breast;
Recepter,erbB-2;
Immuno-histochemistry;
In situ hybridization, chromogenic;
In situ hybridization,fluorescence
- From:
Cancer Research and Clinic
2011;23(2):94-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through comparision of Her-2 gene in invasive breast carcinoma tissues assayed by IHC, CISH and FISH respectively, to explore an optimal detective methods. MethodsIHC, CISH and FISH were used in detection for Her-2 gene of 100 cases, respectively.ResultsCISH identified no Her-2 gene amplification in IHC score negative rumors (6/6) and gene amplication in 7.1%(1/14) of IHC score (+) and in 32 % of IHC score (++) tumors(l6/50) and in 80 % of IHC score (+++) (24/80), Concordant rate between CISH and FISH was 100 %(20/20). ConclusionThere are advantage and disadvantage among IHC,CISH and FISH. CISH is an accurate and dependable methods and would be easy to apply for detection of Her-2 gene.
