Effects of Pseudomonas aeruginosa pvdQ gene on altering antibiotic susceptibility under swarming conditions
10.3760/cma.j.issn.0254-5101.2011.02.008
- VernacularTitle:观察铜绿假单胞菌pvdQ基因对群集游动中抗生素抗性的影响
- Author:
Lili WANG
;
Fengyun GONG
;
Xuhua XIE
;
Jia CHEN
;
Ying SONG
;
Chao XIA
;
Aixia SHEN
;
Mingyou XING
;
Dong XU
;
Jianxin SONG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
pvdQ gene;
Swarming motility;
Antibiotic resistance
- From:
Chinese Journal of Microbiology and Immunology
2011;31(2):129-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether Pseudomonas aeruginosa pvdQ( PA2385 ) gene reveals altered antibiotic susceptibility under swarming conditions. Methods The plasmid pME6032 with pvdQ gene was constructed and identified, then transformed into Pseudomonas aeruginosa PAO1 by the electroporation, building pvdQ overexpression strain. Using the same method building pME6032-PAO1 strain.Bacteria were inoculated in LB overnight , measuring the colony diameter of the swarming zone . Results Strains of pvdQ overexpression was successfully constructed by real-time PCR. Comparison of two strains of the swarming motility of change in diameter: The result showed that PAO1 and pvdQ overexpression strains can both improve the antibiotics resistance. Swarmer cells of pvdQ overexpression strain exhibited a 2- to 4-fold increase in antibiotic resistance toward ceftazidime,ciprofloxacin, meropenem and polymyxin B compared to PAO1 on BM2-swarming agar plates. Conclusion pvdQ gene played an important role in elevating the antibiotics resistance, which through prarticipated in the swarmer cell differentiation.
