Intermedin ameliorates renal injury by inhibition of tubular epithelial cell apoptosis in a renal ischemia/reperfusion rat model
10.3760/cma.j.issn.1001-7097.2011.01.007
- VernacularTitle:垂体中叶素对肾脏缺血再灌注大鼠肾小管上皮细胞凋亡的影响及其机制
- Author:
Xinyan LIU
;
Xinghua LIU
;
Xi QIAO
;
Hong LI
;
Rongshan LI
- Publication Type:Journal Article
- Keywords:
Intermedin;
Kidney;
Reperfusion injury;
Apotosis
- From:
Chinese Journal of Nephrology
2011;27(1):29-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of intermedin (IMD) on tubular cells apoptosis induced by renal ischemia/reperfusion (I/R) injury and its associated mechanism.Methods A total of twenty-four male Wistar rats were randomly divided into four groups (control group, I/R group, empty plasmid group and IMD group). One week after the removal of right kidney, ultrasound plasmid was used to transfect empty or IMD plasmid into the left kidney. Renal I/R model was made by clasping the left renal artery for 45 minutes. Tubular cell apoptosis was determined by TUNEL. Expression of Bax, Bcl-2, Fas was detected by semi-quantitative RT-PCR.Activity of caspase-8 and caspase-9 was evaluated with commercially available kits respectively.Protein level of caspase-3 was measured by Western blotting analysis. Results Compared with control group, apoptosis of tubular epithelial cells, expression of Bax and Fas, activities of caspase-8 and caspase-9, as well as protein level of caspase-3 were all significantly increased in I/R group (all P＜0.05). IMD pre-treatment significantly inhibited all these effects (all P＜0.05). There were no differences of above parameters between empty plasmid group and I/R group. Conclusion IMD pre-treatment protects against renal I/R injury by inhibion of tubular epithelial cell apoptosis.
