IFN-γ up-regulated the expression of BAFF-R in multiple myeloma cells through the NF-κB pathway

VernacularTitle:IFN-γ对多发性骨髓瘤细胞上B细胞活化因子受体表达的影响及相关机制研究
Author: Xianjuan SHEN ; Yueguo WANG ; Xinhua WU ; Hongxiang YUAN ; Wencai ZHU ; Hui CONG ; Huimin WANG ; Shaoqing JU
Publication Type:Journal Article
Keywords: Multiple myeloma; BAFF receptor(BAFF-R); IFN-γ; NF-κB pathway
From: Chinese Journal of Microbiology and Immunology 2011;31(1):20-24
CountryChina
Language:Chinese
Abstract: Objective To investigate the action and mechanism of NF-κB pathway in up-regulating B cell-activating factor receptor (BAFF-R) expression in multiple myeloma cells induced by IFN-γ.Methods Activated NF-κB were detected with Western blot, while the expression of BAFF-R were measured with RT-PCR and ELISA, and investigated the effect of BAY11-7082 on transcription of BAFF-R mRNA and translation of protein in multiple myeloma cells stimulated by IFN-γ. Results IFN-γ can induce the degradation of IκB-α in time-dependent and dosage-dependent manner, and up-regulated BAFF-R expression in multiple myeloma cells. BAY11-7082, an NF-κB inhibitor, inhibited not only the transcription of BAFF-R mRNA but also the protein of regulated by IFN-γin dosage-dependent manner. Conclusion NFκB may play an important role in high expression of BAFF-R in multiple myeloma cells induced by IFN-γ.