SCCmec genotypes of methicillin-resistant Staphylococcus epidermidis in diabetic foot infections
10.3760/cma.j.issn.0254-5101.2011.01.014
- VernacularTitle:糖尿病足感染中耐甲氧西林表皮葡萄球菌SCCmec分型临床研究
- Author:
Qun DING
;
Penghua WANG
;
Yuejie CHU
;
Shuhong FENG
;
Shuyou MENG
;
Qian SUN
;
Daiqing LI
- Publication Type:Journal Article
- Keywords:
Diabetic foot infection;
Methicillin-resistant Staphylococcus epidermidis;
SCCmec genotypes
- From:
Chinese Journal of Microbiology and Immunology
2011;31(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate SCCmec genotypes and drug-resistance profiles of the methieillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from the patients suffered from diabetic foot infections (DFI) in the Tianjin Metabohc Diseases Hospital. Methods After dabridement, specimens of 390 infectious diabetic foot ulcers in the hospital from Jan 2008 to Jun 2010 were collected from the wound basal parts by cotton swab for culture. The disk-diffusion method was performed to examine antimicrobial susceptibility. DNAs of the MRSE strains were extracted, and their SCCmec genotypes were identified by PCR. Results Twenty of the seventy(28.6% ,20/70)Staphylococcus epidermidis strains were mecA posifive. Among the MRSE isolates, 2 ( 10.0% )were SCCmec Ⅱ ,9 (45.0%)were SCCmecⅢ and 9 (45.0%)were SCCmec Ⅳ. None of the isolates were genotyped as SCCmec Ⅰ or Ⅴ. No mater which genotypes they were, all the MRSE isolates were multi-drug resistant. They were resistant not only to β-lactams (including penicillins, cefoxitin and cephems), but also to non-β-lactams (including macrolides, fiuoroquinolones and sulfonamides ) . Resistance to voncomycin and rifampicin were not found in these strains . Conclusion SCCmec Ⅲ and SCCmecⅣ are major genotypes of the MRSE isolates from the infectious diabetic foot ulcers.The SCCmec Ⅳ genotype strains with multi-drug resistant profiles are prevalent in the diabetic foot infections.
