Increased expression of microRNA-146a in peripheral blood mononuclear cells of patients with chronic immune thrombocytopenic purpura and its clinical significance
10.3760/cma.j.issn.0254-5101.2011.01.022
- VernacularTitle:慢性血小板减少性紫癜患者microRNA-146a的表达及其意义
- Author:
Zhijun HAN
;
Zhide HU
;
Anmei DENG
;
Yi SUN
;
Jingbo LIU
;
Yuanlan HUANG
;
Zihe YAN
;
Renqian ZHONG
- Publication Type:Journal Article
- Keywords:
Chronic immune thrombocytopenic purpura;
MicroRNA-146a;
Autoimmune
- From:
Chinese Journal of Microbiology and Immunology
2011;31(1):81-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the increased expression of microRNA-146a(miR-146a) in peripheral blood mononuclear cells (PBMC) of patients with chronic immune thrombocytopenic purpura (ITP) and its clinical significance. Methods Twenty-eight patients with chronic ITP and 28 healthy controls matched with age and gender were enrolled in this study. Fluorescent quantitative PCR reaction was used to detect the relative expression of miR-146a in their PBMC. The serum concentration of TNF-α, IL-2,IL-1 β and IFN-γ were measured by ELISA. CCK-8 method was used to detect the proliferation ability of PBMC , which transfected with miR-146a mimics or inhibitor and then stimulated with platelet . Results The relative expression of miR-146a in ITP patients was higher than that of healthy controls. The increased expression of miR-146a was negatively correlated with the serum TNF-α, IL-2 and IFN-γ. The PBMC transfected with miR-146a mimics had reduced expression of IL-2 and proliferation when stimulated with platelet.In contrast, the opposite effect was observed with the miR-146a inhibitors transfection. Conclusion MiR146a was involved in the pathogenesis of chronic ITP by controlling IL-2 production and PBMC proliferation.Thus, it may be a potential therapy target for chronic ITP.
