Development and validation of HPLC-UV-MS method for the control of four anti-diabetic drugs in suspected counterfeit products.
- Author:
Xiumei DAI
;
Ning AN
;
Jianmin WU
;
Huiyi LI
;
Qiming ZHANG
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2010;45(3):347-52
- CountryChina
- Language:English
-
Abstract:
An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.0) at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four alpha-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.
