Agglutinated activity bioassay method for the determination of antivirus potency of Banlangen granula.
- Author:
Huiying TANG
;
Dan YAN
;
Shaofeng ZHANG
;
Hanbing LI
;
Ronghua LIU
;
Xiaohe XIAO
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2010;45(4):479-83
- CountryChina
- Language:Chinese
-
Abstract:
To establish a bioassay method and quality standard of Banlangen granula, agglutinated activity assay was used in the analysis of the traditional Chinese medicine, Banlangen granula. It showed that masculined effect could be picked up effectively and the products quality of different pharmaceutical factories and different batch numbers from the same factory could be revealed conveniently, accurately, quickly and directly with this method (valence value was between 2 and 11). The established bioassay method had a good reproducibility with RSD = 2%. The dependablity of the activity of red cell agglutination and restrainting influenza virus NA was conspicuous (r2 = 0.878 3). In conclusion, this bioassay method is suitable to control and evaluate the quality of Banlangen granula. Thus the method may provide a simple and effective technique in supervising and examining the quality of other traditional Chinese medicine.
