Determination of serum urea by isotope dilution gas chromatography mass spectrometry:a candidate reference method
- VernacularTitle:血清尿素同位素稀释气相色谱质谱法的建立和研究
- Author:
Tianjiao ZHANG
;
Jiangtao ZHANG
;
Chuanbao ZHANG
;
Haijian ZHAO
;
Jing WANG
;
Jiehong XIE
;
Donghuan WANG
;
Cuihua HU
;
Rong MA
;
Ziyu SHEN
;
Wenxiang CHEN
- Publication Type:Journal Article
- Keywords:
Urea;
Chromatography,gas;
Spectrum analysis,mass
- From:
Chinese Journal of Laboratory Medicine
2008;31(5):536-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a candidate reference method for the measurement of urea in human serum based on isotope dilution/gas chromatography/mass spectrometry.Methods [13C,15N2]Urea used as internal standard Was added to the serum sample and equilibrated with endogenous nonlabeled urea.The serum samples were treated with anhydrous ethanol to emove proteins by precipitation.The serum urea and labeled urea were converted into a trimethylsilyl derivative of 2-hydroxypyrimidine and analyzed by gas chromatography/mass spectrometry system with selected ion monitoring.The concentration of serum ureaWas calculated by the theory of bracketing method.Results The average value of within-run oefficient of vailation(CV),between-run CV and total CV of the procedure were 0.38%(ranged from 0.12%to 0.47%),O.62%(ranged form 0.49% to 0.87%)and 0.73%(ranged from 0.51% to 0.93%).Respectively.The analytical recoveries ranged from 99.37% to 100.95%.The resuhs of analyzing the certified refefence material SRM909b(Level Ⅰand Ⅱ)showed a bias less than 0.2%.Conclusion The procedure for measuring urea in serum is a highly accurate and precise method and can be used as a candidate reference method for serum urea assays.
