Imaging study of interaction between Yersinia pestis and its surface antibody by atomic force microscopy
- VernacularTitle:鼠疫耶尔森菌与表面抗体相互作用的原子力显微镜观测研究
- Author:
Huaqian SUO
;
Kongxin HU
;
Yunqiu YANG
;
Yanfei LIU
;
Qian YUE
;
Jing WANG
- Publication Type:Journal Article
- Keywords:
Atomic force microscopy (AFM);
Yersinia pestis;
Micromorphology
- From:
Chinese Journal of Microbiology and Immunology
2008;28(5):463-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe and compare the atomic force microscopy (AFM) images of Yersinia pestis EV76 and the changes in the morphology of the bacteria treated with normal serum and F1 antibody from rabbit,and to explore the immunoassay method to detect Yersinia pestis by AFM. Methods The Yersinia pestis were treated with normal serum and F1 antibody from rabbit and control buffer. All the prepared samples were observed and analyzed by AFM. The changes in the cell surface structures were probed and characterized through sectional analysis,especially the changes of Ra and Rq value. Results The normal morphology of Yersinia pestis was oval in shape with a relatively smooth surface, the size dimension of which was about 1.1-1.3 μm in length with a section profile of 0.8-1.0 μm in width and 0.04-0.06 μm in step height. The step height of the bacteria treated with the normal serum and F1 antibody was obviously enlarged. The shape of the bacteria treated with F1 antibody changed irregularly. Furthermore, the surface of the bacteria was more roughened. Conclusion The morphological characters of Yersinia pestis has been acquired through its AFM images. The morphology of Yersinia pestis treated with F1 antibody has changed greatly, and the index of roughness can be regarded as the distinguished index to detect Yersinia pestis by AFM.
