The influence of adenovirus latent infection on glutathione and antioxidative enzymes in rat alveolar epithelial cells
- VernacularTitle:腺病毒E1A蛋白对大鼠肺泡上皮细胞谷胱甘肽活性的影响及其机制探讨
- Author:
Yi FANG
;
Bing LI
;
Juan CHEN
;
Qicai LIU
;
Pixin RAN
- Publication Type:Journal Article
- Keywords:
Adenovirus E1A;
Glutathione;
Gamma-glutamylcysteine systhetase (γ-GCS)
- From:
Chinese Journal of Microbiology and Immunology
2008;28(5):416-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the influence of adenovirus latent infection on gamma-glutamylcysteine systhetase(γ-GCS) in rat alveolar epithelial cells. Methods The rat alveolar epithelial cells were stably transfected with the plasmid pE1Aneo and control plasmid. Glutathione(GSH) contents, the activity of γ-GCS were detected in oxidant stress. Then the leuel of protein expression, mRNA expression, and promoter transcriptional activity of glutamate-cysteine ligase catalytic subunit(GCLC) were further detected. Results GSH contents decreased because of adenovirus E1A expression in oxidant stress. E1A repressed the expression and activity of γ-GCS, messenger RNA expression, and promoter transcriptional activity of GCLC. Conclusion Adenovirus E1A decreased the activity of γ-GCS probably by repressed promoter transcriptional activity of GCLC. As a result, GSH contents were downregulated in oxidant stress. Thus Adenovirus latent infection amplified the oxidant/antioxidant imbalance in rat alveolar epithelial cells in oxidants stress, which may be an important mechanism of COPD.
