Effects of hyperbaric oxygen on the expression of MMP-9 and MMP-2 mRNA in the brain tissues and on the permeability of the blood brain barrier after cerebral ischemia and reperfusion
- VernacularTitle:高压氧对缺血再灌注小鼠脑组织MMP-9、MMP-2 mRNA的表达及血脑屏障通透性的影响
- Author:
Hong ZHAO
;
Lina ZHU
;
Jianhua WU
;
Haipeng ZHANG
;
Xuexin CHEN
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Blood-brain barrier;
Ischemia-reperfusion;
Metalloproteinases
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2008;30(5):313-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hyperbaric oxygen(HBO)on the expression of MMP-9 and MMP-2 mRNA in the brain tissues after cerebral ischemia and reperfusion,and the permeability of blood brain barrier(BBB).Methods Using cerebral ischemia-reperfusion models with conscious mice,0.25 MPa(atmosphaera absolutus,ATA)HBO was applied 5 times during the reperfusion period,and 2%Evan's blue(EB)was injected into the tail vein 1 hour before the animals were sacrificed.The expression of MMP-9 and MMP-2 mRNA and EB content were determined by RT-PCR and spectrophotometry.Results The expression of MMP-9 and MMP-2 mRNA aswell as EB content significantly increased in the ischemia-reperfusion group as compared with a sham surgery group.The expression of MMP-9 and MMP-2 mRNA,and EB levels in the HBO group were similar to those in the sham surgery group.The expression of MMP-9 and MMP-2 mRNA and EB levels in the group given HBO plus reperfusion group were significantly decreased as compared with those in the group receiving reperfusion alone. Conclusion HBO can significandy reduce the expression of MMP-9 and MMP-2 mRNA and the permeability of the BBB.
