The effect of ischemic postconditioning on cerebral ischemia-reperfusion injury in diabetic rats
- VernacularTitle:缺血后处理对糖尿病大鼠局灶性脑缺血再灌注损伤的影响
- Author:
Yingyun LU
;
Cuilan WANG
;
Ying LIU
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Ischemic postconditioning;
Cerebral ischemia;
Reperfusion injury;
Cell apoptosis;
Diabetes mellitus
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2008;30(5):316-319
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ischemic postconditioning on cerebral ischemia-reperfusion (I/R)injury in diabetic rats. Methods A rat model of diabetes was established using a single intraperitoneal injection of streptozotocin in 40 male Spragne-Dawley rats.Focal ischemia was induced by transient middle cerebral artery occlusion(MCAO)using a thread.The rats were randomly assigned to a control group,a sham-operated group,an I/R group and an Ⅰ-Post group.The animals in the I/R group were subjected to MCAO for 90 min and then reperfusion.Those in the Ⅰ-post group were subjected to MCAO and 3 cycles of transient ischemia-reperfusion(15 seconds ischemia then 15 seconds reperfusion)before persistent reperfusion.Neurological deficit scores,infarct volume,histological changes in the brain and the number of apoptotic cells were measured 6 hours later.Results There was no significant difference in neurological deficit scores between the I/R group and the Ⅰ-post group.The histological changes and apoptotic cells were significantly less in the Ⅰ-post group compared with the I/R group.Conclusion Ischemic postconditioning can inhibit cell apoptosis and reduce cerebral I/R injury after focal cerebral ischemia-reperfusion in diabetic rats.
