A comparative study on the ways of building the Lewis lung carcinoma animal models
- VernacularTitle:小鼠Lewis肺癌模型制备方法的比较研究
- Author:
Jun ZHAO
;
Jing LU
;
Hongyan YANG
;
Jimin ZHAO
;
Jingming ZHAI
;
Shan LI
;
Xi ZHANG
;
Ziming DONG
- Publication Type:Journal Article
- Keywords:
Carcinoma,lewis lung;
Mice,inbred C57BL
- From:
Cancer Research and Clinic
2008;20(7):439-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the feasibility on building lewis lung carcinoma mouse models through different methods and improve the methods. Methods The method of culture LLC cells in vitro, trypsin digestion method, Ⅳ collagenase method and homogenate method were compared to make the different dose of cell suspension injected into C57BL/6 mice. The feasibility of the improved method was determined through observing the cell count, the tumor formation ratio, the tumor formation time, tumor volume, weight and life habit. Results The method of culture LLC cells in vitro could get needed cells and its tumor formation ratio was 100 %. Trypsin digestion method and homogenate method could get less cells and its tumor formation ratio was about 80 %～90 % and 60 %～75 %. Whereas 1V collagenase method could get most cell count and its tumor formation ratio was 100 %. Conclusion IV collagenase method is a preferred method which is simple,high efficiency and make a strong base on the cancer experimental study.
