The impact of site-directed deglycosylation of the HIV-1 envelope (Env) on its immunogenicity and assembly of functional pseudovirus
- VernacularTitle:HIV-1膜抗原部分糖基化位点改造对免疫原性及假病毒形成的影响
- Author:
Jianhui NIE
;
Chuntao ZHANG
;
Huihui CHONG
;
Chunyu LIU
;
Xueling WU
;
Yu WU
;
Chenyan ZHAO
;
Youchun WANG
- Publication Type:Journal Article
- Keywords:
HIV-1;
Immunogenicity;
Neutralization assay;
Deglycosylation;
ELISPOT
- From:
Chinese Journal of Microbiology and Immunology
2008;28(6):540-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of site-directed deglycosylation of the HIV-1 envelope (Env) on its immunogenicity and assembly of functional pseudovirus. Methods Site-directed deglycosylation were performed using cycling mutagenesis and selection of mutants with DpnⅠ. Single-cycle infection assay was employed to analyze the effect of the mutations on the ability of functional pseudovirus assembly. The influence of deglycosylations on the immunodeficiency of Env was evaluated using pseudovirusbased neutralization assay and ELISPOT assay. Results Mutant N197Q induced higher neutralization activities for both pseudoviruses, but lower Env-specific T-cell response. And N197Q rendered the Env to lose the ability of functional pseudovirus assembly. Mutant G2 induced higher neutralization activities for pseudovirus 74-2 but lower for pseudovirus Wt, and had almost no influence on Env-specific T-cell response and functional pseudovirus forming. Conclusion The site-directed deglycosylation of the HIV-1 Env affects the pseudovirus forming and its immunogenicity.
