Display of heterologous proteins on the surface of E. coli by Red recombineering
- VernacularTitle:应用Red重组工程技术在大肠杆菌外膜展示表达外源蛋白
- Author:
Shanhu LI
;
Qingguo SHI
;
Cuifen HUANG
;
Jianguang ZHOU
- Publication Type:Journal Article
- Keywords:
Recombineering;
Homologous recombination;
Luciferase report gene;
Outer mem-brane protein
- From:
Chinese Journal of Microbiology and Immunology
2008;28(7):656-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective To display of heterologous proteins on the surface of E. coli . Methods The 1653 bp luciferase report gene was knocked in Ipp and ompA genes of chromosome by Red recombine system and selection-counter selection kan/sacB. Results The quantitative analysis results of exogenous lu-ciferase expression displayed that it could be expressed as fusion with the outer membrane proteins on the cell surface. The fusion protein of foreign protein and outer membrane protein Lpp-OmpA-Luc could be high-effi-ciently displayed on cell surface. Conclusion The stable expression of exogenous gene on the surface of E. coli had no effect on the bacterial growth and propagation.
