Causes of reduced expression of Cosmc gene in IgA nephropathy patients
- VernacularTitle:IgA肾病患者Cosmc基因表达低下原因探讨
- Author:
Wei QIN
;
Xiang ZHONG
;
Yingjuan ZHANG
;
Chunyu TAN
;
Liehuan YANG
;
Junming FAN
- Publication Type:Journal Article
- Keywords:
Glomemlonephritis,IgA;
Glycosylation;
Lipopolysacchaxide;
Cosmc gene
- From:
Chinese Journal of Nephrology
2008;24(6):377-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify whether the Cosmc gene down-regnhtion in lgA nephropathy (IgAN) patients is resulted from genetic disorders or external suppressions. Methods Forty IgAN patients, 16 non-IgAN glomerulonephritis patients and 21 healthy controls were enrolled in the study. Genomie DNA was extracted and then Cosmc gene was amplified and sequenced. Peripheral B lymphoeytes were isolated and cultured with RPMI-1640 alone or plus lipopolysaecharide (LPS). The Cosmc mRNA expression levels at baseline, after RPMI-1640 culture or RPMI-1640+LPS treatment were measured respectively by real-time RT-PCR. Results (1) Only 2 missense mutations and 2 silent mutations were detected in coding frame region of Cosine gene in 2 IgAN patients. (2) The baseline Cosmc gene expression level was significantly lower in IgAN patients (31% of that in healthy controls) than that in healthy controls. (3) Relative quantification PCR indicated that Cosmc mRNA expression level was significantly increased (219% of baseline) after RPMI-1640 culture, and treatment of LPS could strongly inhibit this effect. (4) The Cosmc gene expression of healthy control was not affected by RPMI-1640 or LPS. Conclusion It is not genetic disorders but external suppression to cause the down-regulation of Cosmc gene mRNA expression in IgAN.
