The immunoregulatory effects of bone marrow mesenchymal stem cells on allogeneic peripheral B lymphocyte
- VernacularTitle:骨髓间充质干细胞对异体外周血B淋巴细胞的免疫调节作用
- Author:
Lingqi WU
;
Hai BAI
;
Cunbang WANG
;
Xiaoliang YANG
;
Qiang ZHAO
;
Yiwu YANG
;
Mei LIN
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
B lymphocyte;
Immunoglobulin;
Immunoregulatory
- From:
Chinese Journal of Microbiology and Immunology
2008;28(6):486-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunoregulatory effects of bone marrow mesenchymal stem cells (MSC) on allogeneic peripheral B lymphocytes in vitro. Methods MSCs were isolated and cultured from bone marrow by gradient centrifugation. Mononuclear cells were isolated routinely from peripheral blood, then monocytes were eliminated by L-leucine methy ester method. Remained T lymphocytes were eliminated by AET-SRBC rosette method. The action of MSCs and its supernatant on B lymphocytes proliferation in the presence of anti-human IgM goat antibodies (anti-IgM) was investigated by MTT. The IgG, IgM in the supernatant were detected by ELISA. The percent of apoptosis B lymphocytes, co-cultured with MSCs for 24 or 48h, was assayed by FACS. Results MSCs and its supernatant inhibited B lymphocytes proliferation and Ig secretion. The inhibitory effect depended on the amount of MSCs and condition of its supernatant. The date of FACS indicated that the apoptosis ratio of B lymphocytes, co-cultured with MSCs for different times, were non-significant. The inhibitory effect of MSCs on B lymphocytes was temporary and reversible. Conclusion MSCs have immunoregulatory effects on B lymphocytes, and its mechanisms are complex, not only correlating with the concentration of MSCs but also the action between cells and the secretory cytokine of MSCs.
