The polarization and proliferation of TH1 cells inhibited IL-2 through upregulation of SOCS-3 in naive CD4+T cells
- VernacularTitle:IL-2通过上调naive CD4+T细胞SOCS-3表达抑制CD4+T细胞的极化和增殖
- Author:
Yanhui XIE
;
Min WU
;
Tenglong ZHANG
;
Jinhe ZHAO
;
Ying WANG
;
Guowei LIN
- Publication Type:Journal Article
- Keywords:
Interleukin-2;
Suppressor of cytokine signal-3 (SOCS-3);
Naive CD4+T lympho-cyte;
TH1 cell
- From:
Chinese Journal of Microbiology and Immunology
2008;28(7):610-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the polarization and proliferation of naive CD4+TT cells after preincubation with interleukin-2. Methods The expression of suppressor of cytokine signal (SOCS)-3 was detected by the real-time PCR in the naive T lymphocytes of DO11.10 TCR transgenic and C57BL/6N mice after they were preincubated by 50 U/ml of IL-2. The naive CD4+T T lymphocytes preincubated for 4 h by IL-2 were stimulated with ovalbumin (OVA) and inactivated BALB/c spleen cells, respectively. After 14 d, IL-12Rβ1, IL-12β2 and cytoplasm IL-4 (cyIL-4) were detected by flow cytometry. Results SOCS-3 in the naive CD4+T lymphocytes of DO11. 10 TCR transgenic and C57BL/6N mice reached the peak after preincu- bated by IL-2 for 6 h. The polarization to TH1 and the proliferation ability of naive CD4+ T lymphocytes stimulated with specific antigen and allogeneic antigen were inhibited conspicuously during the time of the peak of SOCS-3. Conclusion The expression of SOCS-3 in naive CD4+T lymphocytes can be upregulated by IL-2. The upregulation of SOCS-3 can suppress the polarization to TH1and the proliferation ability of naive CD4+T lymphocytes stimulated by specific antigen and allogeneic antigen.
