Analysis of epidermal growth factor receptor(EGFR) mutations in non-small-cell lung cancer patients in Fujian province
- VernacularTitle:福建省非小细胞肺癌患者EGFR基因突变分析
- Author:
Ling CHEN
;
Yunbin YE
;
Dan CAI
;
Zhilian SHE
;
Xiongwei ZHENG
;
Yanping ZHANG
;
Xiaoling HE
;
Qiang CHEN
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small-ceu lung;
Receptor,epidermal growth factor,Gene;
Mutation
- From:
Cancer Research and Clinic
2008;20(8):509-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the epidemud growth factor receptor(EGFR)mutations in NSCLC patients in Fujian province.Methods Fresh specimens of lung cancer and corresponding normal lung tissue were collected from 50 cases of NSCLC patients.After DNA extraction,nested polymerase chain reaction (nested PCR)and direct deoxyribonucleic acid(DNA)sequencing were used to analyze EGFR gene mutations in NSCLC patients.Results EGFR mutations in tumors were identified from 13 of 50(26%)patients,including 10 cages of in-frame deletion in exon 19 and 3 cases of amino acid substitution in exon 21.Conclusion The mjor type of EGFR mutation in NSCLC patients in Fujian is in-frame deletion in exon 19.
