Electromagnetic fields and the proliferation and matrix synthesis of annulus fibrosus cells
- VernacularTitle:正弦波电磁场对大鼠椎间盘纤维环细胞的生物学影响
- Author:
Tie LIU
;
Hongbo YOU
;
Anmin CHEN
;
Dongming ZHAO
;
Feng LI
- Publication Type:Journal Article
- Keywords:
Electromagnetic fields;
Intervertebral disc;
Annulus fibrosus cells
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2008;30(8):519-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological effects of sinusoidal electromagnetic fields(EMFs)on proliferation and extracellular matrix(ECM)formation by annulus fibrosus(AF)cells in rats.Methods AF cells isolated from rats were randomly divided into a control group and an experimental group.The cells in the experimental group were stimulated with an EMF,while those in the control group were held under the same culture conditions but with no EMF.Flow cytometry and MTT were performed to observe the effects on the ceU cycle and proliferation.Collagen and aggrecan expression were examined after amplification with a reverse transcriptase polymerase chain reaction(RTPCR).Sulfated glycosaminoglycan(sGAG)content wag detected by applying the Alcian blue method. Results AF cell proliferation was not significant until after 4 days of stimulation.Compared with the control group,the expression of type Ⅰ and Ⅱ collagen and Aggrecan were up-regulated,and sGAG content Was increased in the experimental group.Conclusion AF cell proliferation was enhanced by EMF.Gene expression of collagen type Ⅰ and Ⅱ and Aggrecan increased.a8 well as sGAG levels.The results suggest an approach for treating of intervertebral disc degeneration.
