Selection and identification of HLA-A2-restricted CTL epitopes within heparanase
- VernacularTitle:肝素酶HLA-A2限制性CTL表位肽的筛选及其活性鉴定
- Author:
Tong YU
;
Huiju WANG
;
Jianmin YANG
;
Yong FANG
;
Yonglie ZHOU
;
Zaiyuan YE
- Publication Type:Journal Article
- Keywords:
Heparanase;
CTL epitope;
HLA-A2-restricted;
Polypeptide
- From:
Chinese Journal of Microbiology and Immunology
2008;28(8):675-679
- CountryChina
- Language:Chinese
-
Abstract:
Objective To select and identify human leukocyte antigen(HLA)-A2-restricted T-cell epitope within heparanase(Hpa),and to provide aeademic bases for peptide-based immunotherapy with malignant tumor.Methods The Hpa sequence was scanned for prediction of the immunogenic peptides-based CTL epitopes using the HLA-binding prediction software SYFPEITHI and BIMAS.Ten the affinity of candidate epitopes to HLA-A2 was evaluated by T2 binding test.Acfivation of T cell was assessed by ELIS-POT and cytotoxictiy by lactate dehydrogenase(LDH)release assay.Results of the six predicted nona-peptides,Hpa(310-318,FLNPDVLDI)showed high affinity of binding to HLA-A2 and led to IFN-γ secre-tion in vitro.Furthermore,Hpa(310-318) FLNPDVILDI could induce PBMC from a HLA-A2 positive healthy donor to lyse specifically HCC-LM6 and SW-480 cells which expressing both Hpa and HLA-A2.Conclusion The nona-peptide Hpa(310-318)FLNPDVLDI may be all HLA-A2-restricted CTL epitope.which is capable of inducing Hpa-specific CTL in vitro.
