Establishment of antigen capture ELISA method to detect Kaposi's sarcoma-associated herpesvirus antigen
- VernacularTitle:抗原捕获ELISA检测卡波济肉瘤相关疱疹病毒的方法建立
- Author:
Shuihong YAO
;
Qiao TANG
;
Xianfang WANG
;
Huiping QIU
;
Shengqin LI
;
Chun LU
- Publication Type:Journal Article
- Keywords:
Kaposi's sarcoma-associated herpesvirus(KSHV);
Envelope glycoprotein gpK8.1;
Antigen capture;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Microbiology and Immunology
2008;28(8):707-711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish antigen capture ELISA methed to detect Kaposi's sarcoma-as-sociated herpesvirus(KSHV)antigen,and to evaluate its feasibility for clinical application.Methods The BALB/c mice and New Zealand white rabbits were injected with purified recombinant KSHV gpK8.1 proteins to prepare the monoclonal antibody(McAb)and polyclonal antibody(PcAb)anti-gpK8.1,respectively.A new antigen capture ELISA method was established for KSHV antigen detection.The detection reproducibili-ty as well as the sensitivity and specificity of this new assay were determined by the optimization test,which antibody pairs were analyzed to choose the best coating antibody and detecting antibody.The 3 KSHV posi-tive patients sera and 257 patients sera from sexually transmitted disease,cancers or gynecological diseases were detected with this assay to evaluate its value for clinical application.Results When the McAb as coat-ing antibody at concentration of 5 μg/ml and PcAb as detecting antibody at concentration of 1.6μg/ml were selected,the highest P/N value could be obtained.The sensitive analysis of this test could detect recombi-nant KSHV gpK8.1 antigen of 31.28 ng/ml.Meanwhile,it is highly specific to detect KSHV antigen with-out cross reaction to Epstein-Barr vims(EBV),herpes simplex virus(HSV)-1 or HSV-2.All of three KSHV-positive sera and 4 sera from 257 clinical samples were positive with this new assay.which indicated that it could be used for capturing KSHV antigen.Conclusion A sensitive and specific McAb-based anti-gen capture ELISA method to detect KSHV antigen were established successfully.It is of great potential val-ue to develop reagent for KSHV clinical serologic dingnosis.
