Proliferation of antigen specific T cells in vitro and analysis of phenotype and function
- VernacularTitle:抗原特异性T细胞的体外扩增及其表型和效应功能检测
- Author:
Yanjun WANG
;
Daojie LIU
;
Fang LIU
;
Feng REN
;
Bin SUN
- Publication Type:Journal Article
- Keywords:
Adoptive immunotherapy;
Antigen-specific T cells;
Peptide pool
- From:
Chinese Journal of Microbiology and Immunology
2008;28(7):621-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a new peptide-based approach independent of HLA to generate antigen-specific CD+ CD8+T cells. Methods Peripheral blood mononuclear cells(PBMC) were stimula- ted for 6 h with IE-1 peptide pool. Then the activated IFN-γsecreting ceils were tested by immunomagnetic selection. And the selected cells were cultured with radio-inactivated PBMC in medium with 100 IU/ml IL-2 for 4 weeks. Results The generated T cell lines consisted of IE-1 specific CD4+ T (6.88%) and CD8+ T cells 92.99%, which demonstrated antigen-specific killing and cytokine secretion. Conclusion T ceils can be proliferated with this new procedure, and maintain its phenotype and antigen-specific function.
