Expression of Vascular endothelial growth factor mRNA and protein in endothelial progenitor cells in patients during acute cerebral hemorrhage associated with hypertension
10.3760/cma.j.issn.1006-7876.2011.02.007
- VernacularTitle:脑出血急性期内皮祖细胞血管内皮生长因子mRNA及蛋白的表达和分泌增加
- Author:
Wang MIAO
;
Qidong YANG
;
Weixin GUO
;
Xiaoyun XIE
;
Hengfang LIU
;
Hongbo LUO
;
Juan BAO
- Publication Type:Journal Article
- Keywords:
Intracranial hemorrhage,hypertensive;
Endothelium,vascular;
Stem cells;
Vascular endothelial growth factor A
- From:
Chinese Journal of Neurology
2011;44(2):101-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of vascular endothelial growth factor (VEGF)mRNA and protein in endothelia progenitor cells (EPCs) and VEGF in the culture medium and serum of patients during acute period of cerebral hemorrhage associated with hypertension (APCHH). Methods Mononuclear cells from peripheral blood of patients with APCHH ( 16 patients) and hypertension ( 16 patients) were isolated and induced to EPCs. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed to assay VEGF mRNA. VEGF protein was assessed by Western blotting. The VEGF protein level in patient's serum and culture medium ( at day 7 ) were assayed using VEGF ELISA Kit and compared between APCHH group and hypertension group. Results Compared with hypertension group, VEGF mRNA (0. 186 ±0. 035 versus 0.090 ±0.031, t =8.318, P ＜0.0l ) and protein (0. 223 ± 0. 028 versus 0.169 ± 0. 022, t = 3. 744, P ＜ 0. 01 ) expression of EPCs, the concentration of VEGF protein in the supernatant (414 ±37 versus 316 ±29, t =8. 270, P ＜0. 01 ) and in serum (408 ±49versus 222 ±34, t = 12.406, P ＜0. 01 ) were all significantly increased in APCHH group. Conclusion The VEGF protein levels in serum of patients and in the culture medium, VEGF mRNA and protein expression in EPCs were all significantly increased during acute periods of cerebral hemorrhage.
