The role of pituitary adenylate cyclase-activating polypeptide in the growth modulation of human pancreas carcinoma
- VernacularTitle:垂体腺苷酸环化酶激活多肽对胰腺癌细胞的生长调控
- Author:
Min ZHAO
;
Ruolan JIANG
;
Lumei SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Digestion
2001;21(2):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role pituitary adenylate cyclase-activating polypeptide (PACAP) in the growth modulation of PACAP of human pancreas carcinoma cells and determine whether sphingomyolin (SM) may act as a second messenger involved in the postreceptor signal transduction. Methods Human pancreas carcinoma cell strains, JF305, HS766T and ASPC-1 cells were cultivated, reproduced and then treated with PACAP1-38 (10- 12 - 10- 6 M). The amounts of proliferated carcinoma cells were estiimated with Mosmann's method (MTT). The concentrations of intracellular SM in cells were determined with thin layer chromotograph. Intracellular adenosine monophosphate and Ca2 + levels were detected by radioimmunoassay and Fura-2/AM respectively. ResultsIt was found that three kind of human pancreatic cancer cells were proliferated and the intracellular levels of SM, cAMP and cytosolic Ca2+ were increased by treating PACAP1-38. The effect of PACAP1-38 in JF305, HS766T and ASPC-1 could be inhibited by Somatostatin.ConclusionPACAP1-38 may play a role in the proliferation of human pancreatic cancer cells. The postreceptorsignal transduction of PACAP may be mediated by both adenosine cyclinase and Calcium-calmodin pathways. SM may be a second messenger involved in this process.
