The interference of common methods in determination creatinine
- VernacularTitle:常见肌酐测定方法中存在的干扰
- Author:
Lingbo SHI
;
Longshun LIN
- Publication Type:Journal Article
- From:
Chinese Journal of Laboratory Medicine
2001;24(2):102-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interference of common methods in determination of creatinine. Methods Standardized with Fuller′s earth, Jaffe′s method, enzymatic method (ultraviolet), enzymatic method (colour), enzymatic method (electrode) were compared and evaluated. Results In eight interference materials, Jaffe′s method was positively affected by pyruvic acid (≥0.186 mmol/L), α-ketoglutaric acid(≥0.0615 g/L), ascorbic acid(≥0.82 mmol/L) and negatively affected by bilirubin(≥165.5 μmol/L). Enzymatic method (ultraviolet) was not interfered. Enzymatic method (color) was positively affected by creatine(≥0.21 mmol/L) and negatively interfered by dopamine(≥0.28 mmol/L). At last, enzymatic method (electrode) was positively interfered by creatine(≥0.62 mmol/L). Conclusion Enzymatic method(ultraviolet) is the best in the above methods.
