Effect of nuclear factor κB antisense oligodeoxynucleotides on transforming growth factor β1-induced epithelial mesenchymal transition in human renal tubular epithelial cells
- VernacularTitle:核因子κB反义寡核苷酸对转化生长因子β1诱导人肾小管上皮细胞转分化的影响
- Author:
Lei ZHOU
;
Jianqin WANG
;
Zhixia SONG
;
Kuangyun QU
;
Yuke KONG
- Publication Type:Journal Article
- Keywords:
NF-kappa B;
Oligonucleotides,antisense;
Transforming growth factorbeta;
Kidney tubules;
Epithelial cells;
Transdifferentiation
- From:
Chinese Journal of Nephrology
2008;24(7):493-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of nuclear factor κB(NF-κB) antisense oligodeoxynucleotides (AS-ODNs) on transforming growth factor β1 (TGF-β1)-induced epithelial mesenchymal transition (EMT) in human renal tubular epithelial cells. Methods NF-κB AS-ODNs were transferred into the human renal tubular epithelial cells (HK-2), and the cells were stimulated by 10 μg/L TGF-β1 for 24 hours. The expression of NF-κB mRNA and α-SMA mRNA were measured by RT-PCR. α-SMA protein expression was assessed by fluorescence spectrum.Results TGF-β1 significantly up-regulated the expression of NF-κB mRNA, which was 8 folds of blank control (P<0.01). TGF-β1-indueed epithelial mesenchymal transition was inhibited by NF-kB AS-ODN and the NF-KB mRNA expression of AS-ODNs was decreased by 75%(P<0.05).The expression of α-SMA mRNA and protein was also down-regulated obviously (P<0.05).Conclusion NF-κB AS-ODN can inhibit the expression of NF-κB and the epithelial-mesenchymal transition, which may be a new therapeutic strategy against tubulointerstitial fibrosis.
