Studies on the interaction between HSP90 and hepatitis E virus recombinant particle P239
- VernacularTitle:热休克蛋白90与戊型肝炎病毒重组衣壳蛋白P239相互作用的初步研究
- Author:
Zizheng ZHENG
;
Ji MIAO
;
Xiaocheng WU
;
Shuizhen HE
;
Ming TANG
;
Yuanyuan SUN
;
Yingbin WANG
;
Hailian DU
;
Jun ZHANG
;
Ningshao XIA
- Publication Type:Journal Article
- Keywords:
Hepatitis E virus;
HSP90;
Protein-protein interaction
- From:
Chinese Journal of Microbiology and Immunology
2008;28(7):582-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the protein interacting with hepatitis E virus(HEV) recombi-nant capsomeric particles(P239). Methods Protein interacting with HEV was analyzed by the pull-down, MALDI-TOF-MS, co-immunoprecipitation (Co-IP) and CONFOCAL. Results A protein interacting with HEV recombinant particle (P239) was identified as HSP90 by MALDI-TOF-MS. The interaction between HSP90 and P239 was further confirmed by Co-IP. The protein level and localization of HSP90 and P239 in HepG2 were detected. The total quantity of HSP90 didn't change, and the movement of HSP90 from plasma membrane to perinuclei region with P239 was observed. Conclusion HSP90 may play an important role in the trafficking of P239. It suggests that HSP90 participate in the transportation of HEV after infection, which may contribute to the prevention and control of the disease.
