Transfer of regulatory T cells induced by TGF-β1prolonged the skin-graft survival in mice
- VernacularTitle:TGF-β1诱导的调节性T细胞体内输注延长小鼠皮肤移植物存活
- Author:
Jin YUAN
;
Ke WU
;
Wentao HE
;
Feng WANG
;
Zhonghua CHEN
- Publication Type:Journal Article
- Keywords:
TGF-β1;
Regulatory T cell;
Skin transplantation
- From:
Chinese Journal of Microbiology and Immunology
2008;28(8):680-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the probability of transferring the regulatory T cells induced by TGF-β1 to prolong the allograft survival and the mechanisms involved.Methods According to the different culture conditions.three experimental groups were established:control group(T cells from C57 BL/6 mice cultured with II-2),MLR group(T cells from C57BL/6 mice activated by alloantigen)and TGF-βgroup(T cells from C57BL/6 mice activated by alloantigen and cultured with 5.0 ng/ml TGF-β1).After the culture,the ratio of CI4+CD25+T and the Foxq3 expression were measured by FACS and RT-PCR,respectively.On 9th day,the pathologic analysis was performed and the ratios of TH1,TH2 and Treg and the proliferation of lymphocytes were measured.Results The ratio of CD4+CD25+T in TGF-β group was higher than that in control group and MLR group(P<0.05),and Foxp3 was expressed in CD4+CD25+T cell from TGF-βgroup.After transferring ofthe cells,the allografi survival time in TGF-β group was prolonged and its mean survival time(MST)was(22.8±1.9)d,which was longer than that in MLR group and control group (P0.05),lower than that of control group(P<0.05). And the ratio of CD4+CD25+T in TGF-β group was higher than that in control and MLR group obviously(P
