Analysis of an outbreak of nosocomial infection caused by Burkholderia cepacla using Sau-PCR method
- VernacularTitle:Sau-PCR法分析由洋葱伯克霍尔德菌引起的院内感染
- Author:
Xi LU
;
Weimei RUAN
;
Xin XU
;
Shinji YAMASAKI
;
Lei SHI
- Publication Type:Journal Article
- Keywords:
Burkholderia cepacia complex;
Burkholderia infections;
Cross infection;
Polymerase chain reaction;
Sequence homology
- From:
Chinese Journal of Laboratory Medicine
2008;31(9):1047-1050
- CountryChina
- Language:Chinese
-
Abstract:
Objective San-PCR was used to analyze an outbreak of nosocomial infection caused by Brukholderia cepacia.Meanwhile a new DNA amplification technique for genetic fingerprinting-San-PCR was introduced,which owns high sensitivity and facility for homology analysis.Methods The proposed technique was based on the digestion of genomic DNA with the restriction endonuclease Sau3AI and subsequent amplification with primers which carried San3AI recognition site.Finally the homology among the DNA samples was analyzed on the basis of the profiles of agarose gel electrophoresis.Results All of the 11 strains isolated from the patients shclwed the homology except one.The results were confirmed by using PFGE and the results showed consistence with PFGE results.Condusion Sau-PCR is simple,robust,rapid method for DNA fingerprinting with broad perspective.
