Role of Porphyromonas gingivalis invasion on monocyte adhesion to endothelium
- VernacularTitle:牙龈卟啉单胞菌在单核细胞对内皮细胞黏附作用中的影响
- Author:
Dongmei ZHANG
;
Yaping PAN
;
Jian ZHAO
;
Li LIN
;
Yurong KOU
;
Chen LI
- Publication Type:Journal Article
- Keywords:
Porphyromonas gingivalis;
Atherosclerosis;
Intercellular adhesion molecule-l
- From:
Chinese Journal of Microbiology and Immunology
2008;28(8):722-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the role of Porphyromonas P.gingivalis(P.gingivalis)invasion on monocyte adhesion to endothelium and its effect on the intercellular adhesion molecule-1(ICAM-1)produc-tion in endothelial cells.Methods A cell culture model of human umbilical vein endothelial cells (HUVEC) Was used in vitro.The adhesion of monocytes to HUVEC was detected by Rose Bengal staining with or without P.gingivalis invasion.The ICAM-1 mRNA expression in HUVEC Was determined by RT-PCR or quantikine mRNA colorimetric quantification kits.Cell surface protein of ICAM-1 in HUVEC was de-termined by Western blot with or without P.gingivalis invasion.Results The adhesion of monocyte to endo-thelium and expression of ICAM-1(both at mRNA and protein level)were found to be enhanced by P.gingi-valis invasion.Pretreatment of HUVEC with anti-ICAM-l antibody partly abolished the increase in monocyte adhesion to endothelium.Furthermore.the effect of virulent strain W83 was stronger than that of avirulent strain ATCC33277.Condusion P.gingivalis plays an important role in monocyte-endothelium adhesion partly through up regulation of ICAM-1,which may be one of the pathologic mechallisms in atherosclerosis.
