A study on surface plasmon resonance-based gene chip system for rapid pathogen detection
- VernacularTitle:表面等离子体共振型基因芯片系统对临床常见病原微生物的检测
- Author:
Yufeng TIAN
;
Dayong GU
;
Huawei YU
;
Bing HANG
;
Yaou ZHANG
- Publication Type:Journal Article
- Keywords:
Surface plasmon resonance;
Gene-chip;
Pathogen
- From:
Chinese Journal of Laboratory Medicine
2008;31(9):1051-1054
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study application of surface plasmon resonance(SIR)system in detection of clinical pathogen with a gene chip.Methods 27 clinical samples were detected by SPR-based gene chip system.These samples were composed by 8 positive blood samples,3 positive pyoid samples,9 positive leucorrhea samples and positive reproductive tract pyoid samples,1 positive biopsy sample and 6 negative biopsy samples.Specific primers and probes for target pathogens were designed by bioinformatics methods and validated by PCR and enzyme-labelled chemiluminescence,respectively.SPR-based gene chip was prepared and utilized to detect clinical samples by SPR system.Results The primers and probes showed good specificity and accuracy,which can be applied to perform PCR and application of the gene chip.Compared with the clinical analysis,gene chip analysis of 26 clinical samples showed the consistent results.Conclusions SPR detection system proved to be accurate and reliable.The chip will have a promising prospect in application.
