E-cadherin gene hypermethylation and its protein expression in primary adenocarcinomas of the small bowel
- VernacularTitle:原发性小肠腺癌中钙粘蛋白基因异常甲基化及蛋白表达的研究
- Author:
Xiaoyan LI
;
Yuan HUANG
;
Liang GONG
- Publication Type:Journal Article
- Keywords:
Intestinal neoplasms;
Adenocareinomas;
Cadherins
- From:
Chinese Journal of Digestion
2008;28(8):513-516
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the methylation status of E-cadherin(E-cad)and its protein expression in human small bowel adenocarcinomas(SBAC),and the association of these findings with clinico-pathological characteristics.Methods E-cad gene hypermethylation and its protein expression in 36 patients with primary SBAC and the paired adjacent tissues were tested by methylation-specific PCR(MSP)assay and immunohistochemistry(IHC)respectively.Data associated with clinico-pathological characteristics was analyzed.Results The hypermethylation frequency of E-cad was 38.9% in cancerous tissues and 8.3% in adjacent tissues(P＜0.01).The positive rate of E-cad expression was 41.7% in cancerous tissues and 97.2% in adjacent tissues(P＜0.01).Twelve out of 21 cases without protein expression revealed methylated(P＜0.01).Methylation status was closely related to E-cad protein expression in SBAC.The hypermethylation of E-cad gene and the expression Of E-cad protein were significantly correlated with the depth of invasion,the differentiation and lymph node metastasis(P＜0.05),but were not correlated with other clinico-pathologic parameters such as age,sex,tumor size or location(P＞0.05).Conclusions The hypermethylation of E-cad gene can down regulate the protein expression,which closely related to the depth of invasion,the differentiation and lymph node metastasis.
