Gene polymorphism of killer cell immunoglobulin-like receptors and HLA-Cw in patients with ankylosing spondylitis
- VernacularTitle:杀伤细胞免疫球蛋白样受体及HLA-Cw基因多态性与强直性脊柱炎遗传易感性研究
- Author:
Bingchang ZHANG
;
Zhiming LU
;
Yun LIU
;
Yulian JIAO
;
Yueran ZHAO
;
Jianfeng LI
- Publication Type:Journal Article
- Keywords:
Spondylitis ankylosing;
Receptors,immunologic;
HLA antigens;
Histocompatibility antigens class Ⅰ;
Polymorphism,genetic;
Genetic predisposition to diseaoe
- From:
Chinese Journal of Laboratory Medicine
2008;31(10):1114-1118
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alterations in killer cell immunoglobulin-like receptors (KIRs)2D and their specific HLA-Cw ligands in patients with ankylosing spondylitis(AS)and determine whether the changes were correlate to the pathogenesis of AS.Methods Polymerase chain reaction of sequence specific primerB(PCR-SSP)was employed for genotyping the presence or absence of five KIR2D genes(KIR2DL1,2DS1,KIR2DL2,2DL3,2DS2)as well as HLA-Cw01-08 alleles from genomic DNA in 105 individuals with AS,together with 51 individuals with osteoarthritis(OA)and 120 healthy controls.Then HLA-C10-08 was divided into two groups.HLA-Cwasn and HLA-Cwlys to calculate the frequency of KIRID genotype.HLA-Gu alleles and KIR/HLA-Cw genotypes.Results The frequencies of HLA-Cwlys genes were significandy higher in patients with AS(0.269 7)compared with those in OA controls(0.148 2)and healthy controls(0.138 8,P=0.024,P=0.001,respectively).The frequency of KIR2DS1/HLA-Cwlys combination Was also markedly higher in AS group(26.67%)than that in OA controls(11.76%)and healthy controls(13.33%,P=0.039,P=0.018,respectively).Condusion The data suggest that the HLA-Cwlys allele may be associated with genetic susceptibility to AS and moreover.in the existence of HLA-Cwlys.the individuals with KIR2DS1 gene are likely to be at increased risk of AS.
