T-helper epitope peptide improves immunological effects of the B cell epitopes of human heparanase protein
- VernacularTitle:TH线性肽对人肝素酶B细胞表位多抗原肽的免疫增强作用
- Author:
Ling DU
;
Huiju WANG
;
Jianmin YANG
;
Han GAO
;
Yonglie ZHOU
;
Houquan TAO
- Publication Type:Journal Article
- Keywords:
Heparanase;
B cell epitope;
Immune enhancement
- From:
Chinese Journal of Microbiology and Immunology
2008;28(10):869-872
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore available immune strategy to improve immunological effects of the B cell epitopes of human heparanase protein.Methods Based on predicted potential B cell epitopes of human heparanase protein,three candidates of multiple antigenic peptides(MAP)with 8-branches were synthesized and identified by conjugation to antibody against full human heparanase protein.C57BL/6 mice were immunized with the 8-branch MAPs mixed with or without the universal human T-helper IL-1β peptide.The antibody titers of immune serum was assayed by ELISA.Results Most potential epitopes of human heparanase protein were predicted as No.1-15(MAP1),279-293(MAP2)and 175-189(MAP3)in large subunit of human heparanase protein.All three synthesized MAPs mixed with the T-helper epitope induced higher titers of antibodies than them without the T-helper epitope.Conclusion The three B cell epitopes of human heparanase protein could he its B cell preponderant epitopes.The 8-branch MAPs mixed TH cell epitope may be an available and optimizing immune strategy.
