The cytotoxicity of HIV-1 Vpr on the C8166 cells
- VernacularTitle:HIV-1 Vpr蛋白对C8166细胞毒性研究
- Author:
Fang HE
;
Yaoying ZENG
;
Tong WANG
;
Jingxian ZHAO
;
Ning ZANG
;
Changle LIN
- Publication Type:Journal Article
- Keywords:
Recombinant adenovirus;
HIV-1 Vpr;
C8166 cells;
Cell death
- From:
Chinese Journal of Microbiology and Immunology
2008;28(10):899-903
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the T cell cytotoxicity induced by recombinant adenovirus carrying HIV-1 vpr gene.Methods C8166 cells infected with rAd-vpr or negative control rAd-vector,were analyzed for cell cycle distribution and cell death by flow cytometry.The discrimination of living cells,apoptotic and necrotic cells were differentiated with Hoechst-PI double staining under the confocal microscopy.Changes of mitochondrial membrane potential(△ψm)were monitored by JC-1 staining method.Results Annexin V-PI and Hoechst-PI staining indicated the death effects of HIV-1 Vpr on C8166 cells.PI flow cytometric analysis showed that cell cycle arrested in G2 phase.C8166 cell△ψm collapse mediated by Vpr was detected by JC-1 fluorescent staining.Conclusion The ability of recombinant adenovirus carrying HIV-1 vpr gene to induce mitochondria dysfunction,cell cycle G2 phase arrest and cell death was confirmed in C8166 cells.
