Amino acid sequences analysis of human cytomegalovirus UL149 proteins binding peptides
- VernacularTitle:人巨细胞病毒UL149蛋白结合多肽的氨基酸序列分析
- Author:
Yueping WANG
;
Qiang RUAN
;
Yaohua JI
;
Zhengrong SUN
;
Rong HE
;
Ying QI
;
Yanping MA
- Publication Type:Journal Article
- Keywords:
Human cytomegalovirus;
UL149 gene;
Random peptide di8play library;
Screening
- From:
Chinese Journal of Microbiology and Immunology
2008;28(10):904-908
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the peptide that have strong ability binding to HCMV-UL149 encoded protein,and to analyze the characteristics of the amino acid sequence of UL149-binding peptides.Methods Expressed UL149 proteins of three genotypes were used to screen the binding peptide in the random peptide display library,then the encoding sequence of binding peptides in the selected clones were sequenced.The amino acid sequences of the binding peptides were analyzed for their homology,and were com pared with those of the known protein in protein banks.Results The homologous amino acid sequence W/A/F/V-D/E-D/E-G-W/F/I/L were found within the binding peptides selected by proteins of all the three UL149 genotypes proteins,and no difference between three groups was found.The alignment with amino acid sequences of the known proteins in protein banks showed that the binding peptides of UL149 putative protein have homologous amino acid sequences with immunoglobulin heavy chain variable region(IgHV),the serine/threonine protein kinases,compliment factor H,zinc finger protein,MHC Ⅰ molecule,eukaryotic translation initiation factor,nuclear factor and so on.Conclusion The UL149 encoding proteins have binding ability to proteins mentioned above,and might interfere with the immunity responds to HCMV infection through multiple mechanisms.
